In 2017, the total global production of shrimp exceeded 4.3 million tons, an increase of about 7.5% year-on-year, of which the six major producing countries such as China, India, Ecuador, Vietnam, Thailand and Indonesia accounted for more than 80% of global shrimp production. More than 80% of China's farmed shrimp production comes from vannamei shrimp. So far, vannamei shrimp has been developed in China for more than 20 years and has formed a huge industrial chain, but in recent years, South American vannamei shrimp aquaculture diseases have emerged one after another. According to the survey data of the Global Aquaculture Alliance (Figure 1), diseases and farming costs are currently the main difficulties and challenges facing aquaculture in South America worldwide. How to improve the survival rate of vannamei aquaculture and reduce the cost of breeding is a necessary way to improve the international competitiveness of vannamei shrimp farming in China!

With the help of international leading biological fermentation technology, the biochemical division of Xijie Group has developed and produced the whole aerobic fermentation protein powder of single bacillus in China and even the world. Soytide. Quick-benefit peptide is a Bacillus aerobic solid-state fermented soybean meal product, Bacillus fast growth, strong protease secretion ability, is the preferred strain for soybean meal fermentation, Bacillus fermentation product has the characteristics of more thorough degradation of anti-nutritional factors (Figure 2), high crude protein content, high digestion and absorption rate, and good water stability (Figure 3). Quick-in-Peptide contains 107 high-temperature resistant bacillus, which still has viable bacteria after high-temperature granulation or even puffing (Figure 4), and aquatic animals reduce the mortality rate of shrimp by improving their intestinal health, enhancing immunity, improving water quality, and reducing shrimp mortality after feeding. Through the practice of vannamei shrimp farming in South America, we found that the peptide can replace fishmeal in shrimp culture and reduce the cost of farming. Shrimp gut health, reduced mortality, and improved water quality have significant results and are worth trying!

Feasibility study on the replacement of fishmeal with quick-benefit peptides

1. Experimental design

Select 480 healthy vannamei juvenile shrimp, initially weighing 0.5 g, and treat 3 replicates of 40 shrimp per feed. The treatment of juvenile shrimp food included control group (fishmeal group) and three experimental groups (short-acting peptide, short-acting peptide (sterilization) and fermented soybean meal D replacing part of the fishmeal group, respectively), and the feed formula is shown in Table 1. The trial was satiety-fed four times a day for an eight-week trial period. The test environment is 27.0±1 °C, and it is equipped with a seawater circulation device (salinity 33ppt).

Table 1.Test feed composition

2. Test results

2.1 Effect of tachyptic peptide on the growth performance of juvenile shrimp

Regardless of sterilization, Acetin successfully replaced 9 points of fishmeal, among which the non-sterilized Acetin group also significantly increased the weight gain and specific growth rate (P<0.05) of P0.05 compared with other groups, and the sterilized Aceae peptide still improved the production performance of P. vannamei to a certain extent. The results showed that the improvement of the production performance of P. vannamei was due to the degradation of probiotics and product antigens.

2.2 Effect of tachyptic peptide on non-specific immunity of juvenile shrimp

There was no significant difference in superoxide dismutase inhibition between the groups (P>0.05), but lysozyme activity in young shrimp in the quicky-peptide group was significantly higher than that in fishmeal and fermented soybean meal D group (P<0.05). Among them, the lysozyme activity of the instant peptide (sterilized) group was significantly higher than that in the fermented soybean meal D group, and it was also improved to a certain extent compared with the fishmeal group (Figure 6).

Application study of Hyopeptide on survival rate and water quality improvement of P. vannamei

1. Experimental design

120 healthy vannamei shrimp were selected with an initial weight of 4.08+0.12g, 3 replicates per group, 10 juveniles per replicate. The diet treatment was divided into control group, control & probiotics group (control group (+)), fishmeal replacement group (quick peptide group), fish meal replacement & probiotic group (quick peptide (+)), where the probiotics were derived from a commercial brand B (Table 2). The trial was satiety-fed four times a day for four weeks. The test environment is 27.0±2°C, and it is equipped with a seawater circulation device (salinity 33ppt).

Table 2.Experimental design

"X" means not added; "O" means add

2. Test results

2.1 Effect of tachyptic peptide on survival rate of juvenile shrimp

At week 4 of the trial, the replacement of the test water was stopped, and the mortality rate of vannamei vannamei increased in all groups, with the control group having the highest mortality rate of up to 50%, which was significantly higher than that of the other three groups (P<0.05). However, there was no significant difference in the survival rates of the tachytic peptide group, the quick peptide (+), and the control group (+) (P>0.05), and the survival rate of the tachytic peptide group was higher than 60%, which was slightly higher than that of the control group (+) (Figure 5).

2.2 Water transfer effect of tachyptic peptide

After one week, the NO2 concentration in water in the tachyptic peptide group and the tachytic peptide (+) group was significantly lower than that in the control group (P<0.05), and the Bacillus in the tachypeptin group controlled the NO2 concentration in water better than a commercial brand B used in this experiment. The number of bacillus per ml of water in the tachypeptide group and the tachypeptide (+) group at weeks 2 and 4 of the trial was significantly higher than that in the control group (P<0.05).

conclusion

1. The improvement of the production performance of P. vannamei is due to the probiotics and antigen degradation in the product.

2. By improving the water quality environment of vannamei aquaculture, Supenae peptide enhances immunity and improves the survival rate of vannamei shrimp.